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0 and 5 mM MgCl2
• NGS library standards: 10-fold diluted Standard 1-6
T4 DNA Ligase is recombinantly produced in E
The measured Cq and the shape of the amplification curve reflect inhibition
Also in this case the ΔAmp = CqAmplicon1 – CqAmplicon2 can be used as an indicator of RNA integrity
HL SAN Reactions and Format:HL-SAN Triton FREE 25k 0 and 5 mM MgCl2HL SAN Removal of nucleic acids may improve workflows during protein purification, both for laboratory sample preparation and industrial bioprocessing. HL SAN is a nonspecific endonuclease with optimum activity at high salt concentrations. HL SAN is active in a variety of buffers and can be easily inactivated by treatment with a reducing agent. These features make HL SAN particularly useful in the purification of proteins and removal of DNA and RNA
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